Tertiary trisomics, cytogenetical variants with an interchanged chromo
some in addition to the normal chromosome complement, were produced th
rough systematic three-way crosses (primary trisomics/reciprocal trans
locations//normal disomics) in rice (Oryza sativa L.). Twenty-five ter
tiary trisomics were selected on the basis of their morphology and see
d fertility in the F1 progenies of the three-way crosses. They were id
entified by observations of the breeding behavior of their selfed prog
enies and of chromosomal configurations at meiosis. As the tertiary tr
isomics produced in this study are useful for chromosome mapping, addi
tional production of tertiary trisomics is under way.