W. Khunkitti et al., EFFECTS OF BIOCIDES ON ACANTHAMOEBA-CASTELLANII AS MEASURED BY FLOW-CYTOMETRY AND PLAQUE-ASSAY, Journal of antimicrobial chemotherapy, 40(2), 1997, pp. 227-233
The lethal effects of various biocides, in particular two agents (chlo
rhexidine diacetate and polyhexamethylene biguanide) on cysts and trop
hozoites of Acanthamoeba casteilanii have been measured by both plaque
assay and flow cytometry (FCM). Minimum amoebicidal concentrations ag
ainst trophozoites were generally the same by the two methods whereas
minimum cysticidal concentrations were occasionally higher by FCM. Con
centrations of biguanides required to produce an equal reduction in vi
ability were also higher by FCM than by plaque assay.