L-LYSINE PROMOTES THE GROWTH AND SECRETION OF HYBRIDOMA CELL-LINE AE9D6

Authors
DATTA D KUNDU PK SENTHILKUMAR M
Citation
D. Datta et al., L-LYSINE PROMOTES THE GROWTH AND SECRETION OF HYBRIDOMA CELL-LINE AE9D6, Biotechnology techniques, 11(9), 1997, pp. 617-620
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
9
Year of publication
1997
Pages
617 - 620
Database
ISI
SICI code
0951-208X(1997)11:9<617:LPTGAS>2.0.ZU;2-K
Abstract
L-Lysine (10 mu g/ml) promotes significant growth of hybridoma cell-AE 9D6 for up to 9 weeks although mechanism of action was not evident. Ma ximum cell density (> 95% viability) of 1 x 10(8) cells/ml from a seed ing density of 1 x 10(4) cells/ml was obtained within 36 h. Maximum cu mulative monoclonal antibody titre (between 120 h and 240 h) was 2.86 g/l.