OPTIMIZED DETERGENT-BASED METHOD FOR EXTRACTION OF A CHLOROPLAST MEMBRANE-BOUND ENZYME - POLYPHENOL OXIDASE FROM TEA (CAMELLIA-SINENSIS)

Authors
BURTON SG KIRCHMANN S
Citation
Sg. Burton et S. Kirchmann, OPTIMIZED DETERGENT-BASED METHOD FOR EXTRACTION OF A CHLOROPLAST MEMBRANE-BOUND ENZYME - POLYPHENOL OXIDASE FROM TEA (CAMELLIA-SINENSIS), Biotechnology techniques, 11(9), 1997, pp. 645-648
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
9
Year of publication
1997
Pages
645 - 648
Database
ISI
SICI code
0951-208X(1997)11:9<645:ODMFEO>2.0.ZU;2-R
Abstract
The detergent Triton X-114 was used in the successful extraction of a chloroplast membrane-bound plant enzyme, polyphenol oxidase (PPO, E.C. 1.10.3.1), from tea, Camellia sinensis, on a large (20 mg) scale. Tri ton X-100 and Triton X-114 were compared; Triton X-114 gave 4x more ac tivity. Addition of phenol adsorbent PVPP, protease inhibitor PMSF and snail acetone powder cellulases, and purification by hydrophobic chro matography gave 23 mg PPO, 2.73 Units.mg(-1).