Sg. Burton et S. Kirchmann, OPTIMIZED DETERGENT-BASED METHOD FOR EXTRACTION OF A CHLOROPLAST MEMBRANE-BOUND ENZYME - POLYPHENOL OXIDASE FROM TEA (CAMELLIA-SINENSIS), Biotechnology techniques, 11(9), 1997, pp. 645-648
The detergent Triton X-114 was used in the successful extraction of a
chloroplast membrane-bound plant enzyme, polyphenol oxidase (PPO, E.C.
1.10.3.1), from tea, Camellia sinensis, on a large (20 mg) scale. Tri
ton X-100 and Triton X-114 were compared; Triton X-114 gave 4x more ac
tivity. Addition of phenol adsorbent PVPP, protease inhibitor PMSF and
snail acetone powder cellulases, and purification by hydrophobic chro
matography gave 23 mg PPO, 2.73 Units.mg(-1).