KINETIC FLUOROMETRY OF THE IRON-PYOVERDINE COMPLEX IN ACETATE BUFFER MEDIA

In the presence of iron and acetate, the rate of increase of Pseudomon as fluorescens pyoverdine fluorescence was inversely proportional to t he iron concentration, up to approximately 200 mu g/l. An assay based on this observation would however require calibration curves to be gen erated in a matrix similar to that of the sample. Pyoverdine has an af finity for Fe(ll), suggesting that it may be necessary to convert Fe(I I) to Fe(III) prior to assay. More detailed work regarding the mechani sm underlying the change in fluorescence, assay specificity, accuracy and precision is necessary to establish this method as a new analytica l technique.