A DUAL ENZYME AMPEROMETRIC BIOSENSOR FOR MONITORING ORGANOPHOSPHOROUSPESTICIDES

An amperometric biosensor consisting of two enzyme membranes, one a po tato layer rich in acid phosphatase and the other immobilized glucose oxidase membrane, when operated in conjunction with a Clark type disso lved O-2 electrode, detected the pesticide, Paraoxon, at 1 mu g/ml. Th e advantage of this biosensor is that the inhibition of acid phosphata se by the pesticide is reversible and thereby eliminates the problem o f enzyme inactivation and the necessity for its reactivation which is not efficient.