DETERMINING BIOMASS IN VISCOUS CULTURE BROTHS OF SCLEROTIUM-ROLFSII DURING BETA-GLUCAN PRODUCTION - HEXOSAMINE ASSAY AS AN INDIRECT ALTERNATIVE TECHNIQUE

Authors
FARINA JI SINERIZ F MOLINA OE PEROTTI NI
Citation
Ji. Farina et al., DETERMINING BIOMASS IN VISCOUS CULTURE BROTHS OF SCLEROTIUM-ROLFSII DURING BETA-GLUCAN PRODUCTION - HEXOSAMINE ASSAY AS AN INDIRECT ALTERNATIVE TECHNIQUE, Biotechnology techniques, 11(9), 1997, pp. 667-669
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
9
Year of publication
1997
Pages
667 - 669
Database
ISI
SICI code
0951-208X(1997)11:9<667:DBIVCB>2.0.ZU;2-X
Abstract
Micro-Kjeldahl determination of protein content in the cells was not s uitable for the determination of biomass because of the changing compo sition of the cells. The significant correlation was found during cult ivation between the values of the hexosamine assay and those correspon ding to the dry weight method (r(2) = 0.98, slope = 1.04) which showed the validity of both hexosamine and dry weight methods, with the form er being a simple, reliable and not time consuming alternative for the biomass estimation of Sclerotium rolfsii during scleroglucan producti on.