PROCESS MONITORING OF ACETIC-ACID IN ESCHERICHIA-COLI CULTIVATION USING ELECTROCHEMICAL DETECTION IN A FLOW-INJECTION SYSTEM

A flow-injection system for determination of acetic acid in Escherichi a coli cultivations with electrochemical detection based an immobilize d acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) was developed to cope with the problems related to measurements under process conditions such as interferences from pyruvate, drift of electrode baseline and making the cultivation medium and reagents com patible. The results obtained by flow injection analysis were compared with those obtained with an enzymatic test kit.