LIGNIN AND MANGANESE PEROXIDASE-ACTIVITY IN EXTRACTS FROM STRAW SOLIDSUBSTRATE FERMENTATIONS

Authors
CASTILLO MD ANDER P STENSTROM J
Citation
Md. Castillo et al., LIGNIN AND MANGANESE PEROXIDASE-ACTIVITY IN EXTRACTS FROM STRAW SOLIDSUBSTRATE FERMENTATIONS, Biotechnology techniques, 11(9), 1997, pp. 701-706
Citations number
23
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
11
Issue
9
Year of publication
1997
Pages
701 - 706
Database
ISI
SICI code
0951-208X(1997)11:9<701:LAMPIE>2.0.ZU;2-D
Abstract
Manganese and lignin peroxidase (MnP, LiP) activities were measured in straw extracts from cultures of Phanerochaete chrysosporium. Out of s ix MnP substrates, the MBTH/DMAB (3-methyl-2-benzothiazolinone hydrazo ne/3-(dimethylamino)benzoic acid), gave the highest MnP activity. Dete ction of LIP activity as veratryl alcohol oxidation was inhibited by p henols in the straw culture extracts. Appropriate levels of veratryl a lcohol and peroxide (4 mM and 0.4 mM, respectively), and a restricted sample volume (not larger than 10%) were necessary to detect activity.