FLOW CYTOMETRIC ANALYSIS ON REACTIVITY OF HUMAN T-LYMPHOCYTE-SPECIFICAND CYTOKINE-RECEPTOR-SPECIFIC ANTIBODIES WITH PERIPHERAL-BLOOD MONONUCLEAR-CELLS OF CHIMPANZEE (PAN-TROGLODYTES), RHESUS MACAQUE (MACACA-MULATTA), AND SQUIRREL-MONKEY (SAIMIRI-SCIUREUS)

There are relatively few monoclonal antibodies (mAb) that have been ch aracterized for their applicability in studies on the immune system of various nonhuman primates. In the present study, we identified a larg e number of mAb that can be used in future immunological studies in th ree different nonhuman primates, i.e., chimpanzees, rhesus macaques, a nd squirrel monkeys. The reactivity of 161 anti-human mAb to T-cell an tigens and cytokine receptors were tested on peripheral blood mononucl ear cells (PBMC) from the three primate species by flow cytometric ana lysis. A total of 105 (65%), 73 (45%), and 68 (42%) antibodies reacted with PBMC from chimpanzees, rhesus macaques, and squirrel monkeys, re spectively. Out of the 161 mAb, 38 reacted with all three species and 112 reacted with one or two of the species. No specific reaction was o bserved with mAb to receptors to GM-CSF, 4-1BB, FLT3, FLX2, common bet a-chain, IL-1 (type I receptor), and IL-8.