A NITRIC OXIDE-RELEASING REAGENT, S-NITROSO-N-ACETYLPENICILLAMINE, ENHANCES THE EXPRESSION OF SUPEROXIDE DISMUTASES MESSENGER-RNA IN THE MURINE MACROPHAGE CELL-LINE RAW264-7

Authors
SANO H HIRAI M SAITO H NAKASHIMA I ISOBE KI
Citation
H. Sano et al., A NITRIC OXIDE-RELEASING REAGENT, S-NITROSO-N-ACETYLPENICILLAMINE, ENHANCES THE EXPRESSION OF SUPEROXIDE DISMUTASES MESSENGER-RNA IN THE MURINE MACROPHAGE CELL-LINE RAW264-7, Immunology, 92(1), 1997, pp. 118-122
Citations number
21
Categorie Soggetti
Immunology
Journal title
Immunology → ACNP
ISSN journal
00192805
Volume
92
Issue
1
Year of publication
1997
Pages
118 - 122
Database
ISI
SICI code
0019-2805(1997)92:1<118:ANORSE>2.0.ZU;2-Y
Abstract
Murine interferon-gamma (IFN-gamma) stimulates the murine macrophage t umour cell line RAW264-7 to produce nitric oxide (NO). IFN-gamma induc es expression of inducible NO synthase (iNOS), manganese superoxide di smutase (Mn-SOD) and copper zinc SOD (CuZn-SOD) in these cells. To inv estigate the mechanism of induction of SOD expression, we added S-nitr oso-N-acetyl penicillamine (SNAP) to RAW264-7 cells. SNAP enhanced the expression of Mn-SOD and CuZn-SOD. These results suggest that when pr oducing NO, RAW264-7 cells express SOD that might protect them from NO toxicity.