PROMOTERS FROM DROSOPHILA HEAT-SHOCK-PROTEIN AND CYTOMEGALOVIRUS DRIVE TRANSIENT EXPRESSION OF LUCIFERASE INTRODUCED BY PARTICLE BOMBARDMENT INTO EMBRYOS OF THE OYSTER CRASSOSTREA-GIGAS
Jp. Cadoret et al., PROMOTERS FROM DROSOPHILA HEAT-SHOCK-PROTEIN AND CYTOMEGALOVIRUS DRIVE TRANSIENT EXPRESSION OF LUCIFERASE INTRODUCED BY PARTICLE BOMBARDMENT INTO EMBRYOS OF THE OYSTER CRASSOSTREA-GIGAS, Journal of biotechnology, 56(3), 1997, pp. 183-189
Using high velocity particle bombardment, we transferred a reporter ge
ne into early stages of the oyster Crassostrea gigas and showed the ex
pression of the introduced genes in these embryos at later stages of d
evelopment. We tested two promoters: (1) the heat shock protein 70 pro
moter of Drosophila; (2) the cytomegalovirus early promoter, both link
ed to the luciferase reporter gene. The hsp70-luc (pDrluc) construct a
llowed an expression level up to 55-fold higher than the control in a
heat inducible fashion. The CMV-luc (pCMVL) construct constitutively g
ave a 4-fold higher expression than the control. This confirms the sui
tability of particle bombardment for transfecting genes into eggs, zyg
otes and trochophores of bivalves and demonstrates the functionality o
f two heterologous expression vectors in C. gigas. (C) 1997 Elsevier S
cience B.V.