DETERMINATION OF ACYCLOVIR IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION - METHOD DEVELOPMENT AND METHOD VALIDATION

Authors
SMIDOVNIK A WONDRA AG PROSEK M
Citation
A. Smidovnik et al., DETERMINATION OF ACYCLOVIR IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION - METHOD DEVELOPMENT AND METHOD VALIDATION, HRC. Journal of high resolution chromatography, 20(9), 1997, pp. 503-506
Citations number
13
Categorie Soggetti
Chemistry Analytical
Journal title
HRC. Journal of high resolution chromatography → ACNP
ISSN journal
09356304
Volume
20
Issue
9
Year of publication
1997
Pages
503 - 506
Database
ISI
SICI code
0935-6304(1997)20:9<503:DOAIPB>2.0.ZU;2-3
Abstract
A HPLC method for the determination of acyclovir in plasma is describe d. The method is simple and sensitive enough for bioequivalence studie s, where a large number of plasma samples with low acyclovir concentra tion are involved. The procedure is based on the deproteinization of p lasma with perchloric acid and separation of acyclovir on a Hypersil O DS column at pH 5.6 with UV detection. The calibration standards are l inear up to at least 4000 ng/mL and the limit of quantification is 10 ng/mL.