IN-VITRO IMMUNOSTIMULATION OF TURBOT (SCOPHTHALMUS-MAXIMUS) LEUKOCYTES WITH BETA-GLUCAN AND OR PHOTOBACTERIUM-DAMSELA BACTERIN/

Respiratory burst and phagocytic activities of turbot (Scophthalmus ma ximus) leucocytes were determined after an in vitro incubation of head kidneys with different doses of yeast glucan and Photobacterium damse la (formerly Vibrio damsela) O-antigen. Although there was an incremen t of these immune functions with the treatment of glucans and O-antige n compared with controls, they were inhibited at high doses (200 mu g/ ml). The optimum dose of glucan was 50 mu g/ml when it was administere d alone, but a concentration of 10 mu g/ml was enough to produce the s timulatory effect.