INTERFERENCES OF O-RAFFINOSE CROSS-LINKED HEMOGLOBIN IN 3 METHODS FORSERUM CREATININE

The interferences of o-raffinose cross-linked hemoglobin (Hemolink(TM) ) were examined and compared in two serum creatinine methods on the Hi tachi 717 [Boehringer Mannheim (BMC) and Synermed] and in an enzymatic creatinine method on the Vitros 750 (Johnson & Johnson). Interference was considered significant when the change in creatinine concentratio n from the control exceeded the 95% confidence limits of each method. Significant interference was observed for the BMC/Hitachi 717 method w ith Hemolink greater than or equal to 5 g/L. No interference was obser ved for the Synermed/Hitachi 717 assay with Hemolink up to 30 g/L in n ormal samples and up to 50 g/L in samples with increased creatinine. N o interference was observed for the Vitros 750 assay with Hemolink up to 50 g/L at both normal and increased concentrations of creatinine. A lthough the BMC/Hitachi 717 method was considered unacceptable, the Sy nermed/Hitachi 717 and the Vitros 750 methods allow accurate quantific ation of serum creatinine in the presence of Hemolink.