A RADIATION HYBRID MAP OF HUMAN-CHROMOSOME-5 WITH INTEGRATION OF CYTOGENETIC, GENETIC, AND TRANSCRIPT MAPS

One of the major goals of the human genome project is to establish a p hysical map of each human chromosome with a density of sequence-tagged site (STS) markers exceeding one every 100 kb. We report here the gen eration of a human chromosome 5-specific radiation hybrid CRH) map tha t includes 556 markers. Of these markers, 132 loci are ordered with a maximum likelihood ratio of >1000:1 compared with the next most likely order. An additional 113 loci were ordered relative to these backbone markers with a maximum likelihood ratio of >10:1 but <1000:1. Togethe r, these 245 loci form an ordered framework map for the chromosome. Us ing this framework, >300 more markers were localized based on two-poin t analysis with the ordered set. On average, there are 50 markers in c ommon with the RH map presented here and other chromosome 5 maps inclu ded in the current whole genome cytogenetic, genetic, and physical map s. The accuracy of all the maps is evident in that there are no more t han two discrepancies between any one of them and these data. All of t he maps encompassing chromosome 5 complement each other providing exce llent STS coverage with >2200 loci combined. The chromosome 5-specific RH map contains 20% of these independent loci. In addition, our RH ma p contains STSs derived from clones suitable for fluorescent in situ h ybridization, allowing alignment to the cytogenetic map. Together, the se maps will assist in the assembly of sequence-ready contigs and will aid in the identification of disease loci on chromosome 5 by position al cloning and positional candidate approaches.