M. Dumonceaux et al., CLONING OF THE ANTIGEN 85A FROM MYCOBACTERIUM-GORDONAE AND ITS USE FOR THE SPECIFIC PCR IDENTIFICATION OF THESE MYCOBACTERIA, Molecular and cellular probes, 11(4), 1997, pp. 251-258
Citations number
44
Categorie Soggetti
Cell Biology",Biology,"Biochemical Research Methods
The complete nucleotide sequence of 85A antigen of Mycobacterium gordo
nae was determined. This gene encodes 339 amino acids, including 43 am
ino acids for the signal peptide, followed by a mature protein of 296
amino acids. A polymerase chain reaction (PCR) assay for the rapid det
ection of M. gordonae DNA using two pairs of oligonucleotide primers,
derived from our sequence, is described. This one-step PCR has been us
ed successfully to amplify 38 strains of M. gordonae. Conversely, the
primers did not amplify DNA from any of the 25 mycobacterial species t
ested. The results suggest that this PCR assay could be a good alterna
tive to existing commercial assays for the specific identification of
M. gordonae on early culture on solid medium or on early BACTEC broth
culture. (C) 1997 Academic Press Limited.