We demonstrate the use of combined SSCP and heteroduplex analysis in t
he detection of PAX6 mutations using non-radioactive silver staining.
A panel of aniridia patients was screened by this approach and we show
that a greater number of mutations was detected than would have been
found by running each technique alone. Six previously unreported aniri
dia mutations in PAX6 are also described. (C) 1997 Academic Press Limi
ted.