USE OF SPECTRAL MAPPING TECHNIQUE FOR THE SEPARATION OF SOLVENT STRENGTH AND SELECTIVITY ON BETA-CYCLODEXTRIN POLYMER-COATED HPLC COLUMN USING MONOAMINE-OXIDASE INHIBITORY DRUGS AS SOLUTES

Spectral mapping technique has been used for the separation of the str ength and selectivity of retention of 17 monoamine oxidase inhibitory drugs and 5 organic modifiers on a beta-cyclodextrin polymer-coated si lica column (beta CDP). The potency values of the spectral maps were c onsidered as indicators of the capacity of drugs to bind to the beta C DP surface and the capacity of organic modifiers to remove the drugs f rom the surface. The multidimensional selectivity map were visualized by the two-dimensional nonlinear mapping technique. Stepwise regressio n analysis established that both the steric dimensions and hydrophobic ity of drugs influence the strength and selectivity of their binding t o beta CDP whereas the selectivity of organic modifiers depended on th e bulkiness of the molecule. The data suggest that the interaction of drugs and organic modifiers with the surface of the beta CDP support d epends on their capacity to fit sterically in the beta CDP cavity and the strength of the hydrophobic forces between the apolar inner wall o f the cyclodextrin cavity and the substructures of guest molecules ins erted in the cavity.