SYNTHESIS OF POLYMER-COATED MIXED-FUNCTIONAL PACKING MATERIALS FOR DIRECT ANALYSIS OF DRUG-CONTAINING SERUM AND PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
T. Kanda et al., SYNTHESIS OF POLYMER-COATED MIXED-FUNCTIONAL PACKING MATERIALS FOR DIRECT ANALYSIS OF DRUG-CONTAINING SERUM AND PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 672(1-2), 1994, pp. 51-57
Silicone polymer-coated mixed-functional (PCMF) silica packing materia
ls were developed for the direct determination of drugs contained in s
erum or plasma. The new stationary phases were prepared by the followi
ng three-step procedure: (1) coating porous silica with a silicone pol
ymer; (2) partial introduction of hydrophobic groups; and (3) introduc
tion of hydrophilic groups. Two PCMFs were synthesized, one having pol
yoxyethylene groups as hydrophilic groups (PCMF-POE) and the other hav
ing oligoglyceryl groups (PCMF-OG). PCMF-POE showed higher recoveries
for injected proteins and a greater overall retention for drug molecul
es than PCMF-OG. PCMF-POE did not show any column deterioration in 500
serum sample injections (10 mi in total).