DETERMINATION OF STEROL CONTENT IN DIFFERENT FOOD SAMPLES BY CAPILLARY GAS-CHROMATOGRAPHY

Citation
M. Rodriguezpalmero et al., DETERMINATION OF STEROL CONTENT IN DIFFERENT FOOD SAMPLES BY CAPILLARY GAS-CHROMATOGRAPHY, Journal of chromatography, 672(1-2), 1994, pp. 267-272
Citations number
20
Categorie Soggetti
Chemistry Analytical
Journal title
Volume
672
Issue
1-2
Year of publication
1994
Pages
267 - 272
Database
ISI
SICI code
Abstract
An accurate method for the determination of sterols by capillary gas c hromatography was developed and applied to the analysis of food. The p rocedure includes the following steps: dichloromethane-methanol (2:1, v/v) lipid extraction, saponification at 80 degrees C and separation o f the unsaponifiable matter with cyclohexane, derivatization to form t rimethylsilyl ethers and gas chromatography using 5 alpha-cholestane a s the internal standard. The method shows good accuracy, precision and sensitivity and is suitable for the determination of sterols in food.