E. Ong et al., CLONING AND SEQUENCE-ANALYSIS OF 2 LACCASE COMPLEMENTARY DNAS FROM THE LIGNINOLYTIC BASIDIOMYCETE TRAMETES-VERSICOLOR, Gene, 196(1-2), 1997, pp. 113-119
Laccases are oxidoreductase enzymes involved in the oxidation of vario
us phenolic compounds. They may play a role in the biodegradation of l
ignin and in the dechlorination of chlorophenols. The cDNAs encoding l
accase LccI and a putative laccase LccIV and the gene for LccI from th
e white-rot basidiomycete Trametes versicolor were cloned, sequenced a
nd characterized. The genomic DNA of lccI consists of 2128 bp, with th
e coding region interrupted by 10 introns; the cDNA consists of a 1560
bp open reading frame (ORF). The cDNA of the putative lccIV gene cons
ists of a 1581 bp ORF, with a 794 bp 5' untranslated region. The size
of the major transcript for both lccI and lccIV is approximately 2.3 k
b. Transcription of lccIV was induced by 2,5-dimethylaniline, whereas
the opposite effect was observed for lccI. Laccases I and IV contain h
ighly conserved histidinyl and cysteinyl residues, believed to be invo
lved in binding copper, and share extensive sequence similarity with o
ther laccases produced by both ligninolytic and non-ligninolytic fungi
. (C) 1997 Elsevier Science B.V.