SEPARATION AND DETERMINATION OF THE PRODUCTS OF GUM HYDROLYSIS BY THIN-LAYER CHROMATOGRAPHY

A method for identifying gums by thin-layer chromatography on KSKG sil ica-gel plates impregnated with phosphate buffer solutions was develop ed. Effects of the pH of buffer solutions and the nature of the cation (K+ or Na+) on the separation of gum monosaccharides were examined. M obile phases containing polar aprotic solvents were suggested. Neutral sugars and uronic acids can be simultaneously separated with the use of these mobile phases. A modified reagent was used for the visualizat ion of sugars. It produces different colors of chromatographic zones a nd retains its properties on long storage.