Rm. Wooten et al., EFFECTS OF OLEIC-ACID ON MURINE CD4(-CELL DEATH AND ANTI-CD3 OR SUPERANTIGEN INDUCED PROLIFERATION AT LOW-TEMPERATURE() T), Developmental and comparative immunology, 21(4), 1997, pp. 375-384
Studies were conducted to examine the effects of low, yet physiologica
lly relevant, temperatures on murine T lymphocyte responses. Akin to e
ctothermic vertebrate responses, lectin-induced murine T cell prolifer
ation was previously shown to be ablated at temperatures 10 degrees C
below optimal (i.e. 27 degrees C); responsiveness at 27 degrees C was
restored by the addition of oleic acid (18:1). The aim of the present
study was to address the mechanism involved in such low temperature su
ppression. Murine splenic CD4(+) T lymphocytes stimulated with either
alpha CD3 or SEB exhibited cell death, as opposed to anergy, at 27 deg
rees C. However proliferation was observed in the presence of 18:1. Th
us low temperature-suppression of murine CD4(+) T cells is also mediat
ed through TCR and/or CD3 pathways. Additional studies examining the t
emporal effects of adding 18:1, as well as temperature shifts, indicat
ed that the cell death induced by stimulation at low temperature was p
reventable by 18:1. (C) 1997 Elsevier Science Ltd.