Objective-The objective of this study was to determine the effectivene
ss of a 5-minute surgical scrub using either a one-brush or a two-brus
h technique in clean and dirty surgical procedures, and to compare the
efficacy of povidone iodine with chlorhexidine as surgical scrub solu
tions. Study Design-Prospective clinical trial. Methods-Nine veterinar
ians scrubbed their hands on eight separate occasions using either pov
idone iodine or chlorhexidine gluconate. A 5-minute scrub and either a
one-brush or two-brush technique used in both clean and dirty operati
ons were evaluated by taking glove juice samples before scrubbing, imm
ediately after scrubbing, and 30, 60, 90, and 120 minutes after scrubb
ing. Glove juice samples were cultured and the colonies were counted.
Percent reductions of bacterial forming units were calculated for all
eight scrub procedures. Results-All scrub procedures provided an adequ
ate percent reduction in colony forming units (CFU) during the 2-hour
sampling period. The number of CFU immediately after scrubbing were si
gnificantly lower than prescrub. At 120 minutes, there were significan
tly fewer CFUs than presecrub, but there were more than immediately af
ter scrubbing. No significant difference in reduction in CFUs were det
ected between one-brush and two-brush technique. Both chlorhexidine an
d povidone iodine scrub solutions adequately reduced bacterial colony
counts for 120 minutes after scrubbing regardless of the amount of con
tamination before skin preparation. Conclusions-Bacterial counts after
a hand scrub procedure using a one-brush technique were not significa
ntly different than after a procedure that used a two-brush technique.
Povidone iodine and chlorhexidine are equally effectively in decreasi
ng bacterial numbers on the skin, given a variety of contamination lev
els present before the scrub procedure. Clinical Relevance-Surgeons ma
y use either chlorhexidine or povidone iodine for antiseptic preparati
on of their hands before surgery. A two-brush technique is not necessa
ry. (C) Copyright 1997 by The American College of Veterinary Surgeons.