GRADIENT, HIGH-RESOLUTION, MAGIC-ANGLE-SPINNING NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY OF HUMAN ADIPOCYTE TISSUE

The recently developed technique of gradient, high-resolution magic-an gle spinning NMR (g-hr-MAS-NMR) spectroscopy was applied to the study of ex vivo human lipoma and liposarcoma tissue. Compared with conventi onal H-1-NMR, the g-hr-MAS method yielded a large improvement in spect ral resolution and permitted the detection of metabolite resonance's i n a well-differentiated liposarcoma that was not observed in spectra o f similar samples obtained using nonspinning NMR methods. These findin gs suggest that g-hr-MAS-NMR spectroscopy provides a key improvement i n spectral quality for ex vivo lipoma and liposarcoma tissue thereby p ermitting a more precise determination of tissue metabolite compositio n than conventional nonspinning NMR methods.