RADIOLABELING OF HUMIC MATERIAL BY ENZYMATICALLY MEDIATED INCORPORATION OF C-14 PHENOL

This paper discusses the production of C-14-labelled sodium humate by horseradish peroxidase mediated incorporation of C-14-phenol. The use of solubilised horseradish peroxidase and immobilised horseradish pero xidase during labelling is compared. C-14-polyphenol may be produced d uring the reaction but is reduced to 2% of the total radioactivity whe n the immobilised form of the enzyme is used in the reaction. The radi olabelled material is stable with respect to time and migration throug h sand. The mobility of the radiolabelled humic material through sand columns is investigated and is shown to be dependent on the production route. Radiolabelled humic prepared using the immobilised enzyme exhi bits enhanced mobility through sand columns compared to radiolabelled humic prepared using either the soluble form of the enzyme or C-14-met hylamine to radiolabel the humic. (C) 1997 Elsevier Science Ltd.