A. Dieffenbacher et P. Dysseler, THE DETERMINATION OF TRANS UNSATURATED FATTY-ACIDS IN EDIBLE OILS ANDFATS BY CAPILLARY GAS-LIQUID-CHROMATOGRAPHY, Pure and applied chemistry, 69(8), 1997, pp. 1829-1837
Collaborative studies on the application of capillary gas-liquid chrom
atography for the determination of trans unsaturated fatty acids are d
escribed. The method investigated involves preparation of methyl ester
s with methanolic potassium hydroxide followed by analysis of fatty ac
id methyl esters by gas-liquid chromatography on polar capillary colum
ns. Care has to be taken in selecting working conditions to obtain the
best obtainable resolution of signals necessary for reliable identifi
cation and determination of trans unsaturated fatty acids.