LANGMUIR-BLODGETT-FILMS COMPOSED OF MONOLAYERS OF AMPHIPHILIC MOLECULES AND ADSORBED SOLUBLE-PROTEINS

Langmuir-Blodgett (LB) films with the structure, monolayer of surfacta nt 1/adsorbed protein layer/monolayer of surfactant 2, were deposited and studied by ellipsometry, IR spectroscopy and atomic force microsco py (AFM) techniques. To avoid protein denaturation, a new method of de position was applied in which protein molecules avoided contact with t he air-water interface and were adsorbed onto the hydrophilic surface of the deposited monolayer in optimal conditions. Five surfactants, cy tochrome c, alcohol dehydrogenase and glutathione S-transferase (GST) were used in these structures. The results of measurements on the enzy matic activity of GST show that soluble proteins can be included in LB films using the proposed method without considerable denaturation.