MOLECULAR FILM ASSEMBLY VIA LAYER-BY-LAYER ADSORPTION OF OPPOSITELY CHARGED MACROMOLECULES (LINEAR POLYMER, PROTEIN AND CLAY) AND CONCANAVALIN-A AND GLYCOGEN

Protein multilayer assemblies were prepared by two different methods: (1) alternate adsorption of oppositely charged polyions; (2) alternate adsorption with specific enzyme/substrate interaction. The formation of alternate outermost layers (opposite charge or opposite specificity ) ae each adsorption cycle is the key point of the procedure. Three mo des of polyion film assembly were demonstrated: (a) alternation of lin ear polyanions and polycations; (b) insertion of charged particles in the multilayer at every other step (twelve different globular proteins were assembled alternating with Na (poly( styrenesulfonate), poly(eth yleneimine)) or Cl (poly (diallyldimethylammonium))); (c) alternation of polyions and preformed charged plates: delaminated montmorillonite clay. Specific interaction was obtained between consecutive layers of glycogen and concanavalin A. Concanavalin A possesses four binding sit es for its ligand D-glucose and allows the binding of glycogen (branch ed poly (glucose)) layers on both sides of the protein.