The polyhedrin gene (polh) of a characteristically distinct Spodoptera
litura nuclear polyhedrosis virus isolate (SIMNPV) is identified in t
he HindIII-F fragment of the viral DNA. The nucleotide sequence of the
1057 base pair (bp) region of this fragment contains an open reading
frame (ORF) without any intervening sequence for coding a polypeptide
of 246 amino acids. Analysis of the nucleotide sequence and deduced am
ino acid sequence indicate that this has more than 70% sequence identi
ty to known polyhedrins, The coding region is preceded by an AT rich r
egion containing the conserved late promoter motif TAAG. The upstream
promoter and coding regions of this polh gene are more similar to polh
of the NPVs of Spodoptera frugiperda (Sf), Spodoptera exigua (Se) and
Panolis flamea (Pf).