GROWTH-MECHANISM OF THE (110) FACE OF TETRAGONAL LYSOZYME CRYSTALS

The measured macroscopic growth rates of the (110) face of tetragonal lysozyme show an unexpectedly complex dependence on the supersaturatio n. In earlier studies it has been shown that an aggregate growth unit could account for experimental growth-rate trends. In particular molec ular packing and interactions in the growth of the crystal were favore d by completion of the helices along the 4(3) axes. In this study the molecular orientations of the possible growth units and the molecular growth mechanism were identified. This indicated that growth was a two -step process: aggregate growth units corresponding to the 4(3) helix are first formed in the bulk solution by stronger intermolecular bonds and then attached to the crystal face by weaker bonds. A more compreh ensive analysis of the measured (110) growth rates was also undertaken . They were compared with the predicted growth rates from several disl ocation and two-dimensional nucleation growth models, employing tetram er and octamer growth units in polydisperse solutions and monomer unit s in monodisperse solutions. The calculations consistently showed that the measured growth rates followed the expected model relations with octamer growth units, in agreement with the predictions from the molec ular level analyses.