An experiment is described in which the subjective depth-of-focus (DOF
) of the eye, defined as the range of focusing errors for which the im
age of the target appears to have the same clarity, contrast, and form
as the optimal in-focus image, was measured as a function of the size
of high contrast (99%) Snellen Es for 5 trained subjects under cyclop
legia. Mean DOF increased by approximately 60% as the size of the lett
er detail increased from -0.2 to 0.87 log min are (Snellen equivalent:
6/3.8 to 6/45), although there were considerable intersubject variati
ons. DOF declined with increasing pupil diameter, the mean total DOFs
being 0.86, 0.59, and 0.55 D for 2-, 4-, and 6-mm pupils, respectively
, In a second experiment, use of low (21%) contrast letters with a 4-m
m pupil and 4 subjects marginally increased the DOF (by 0.08 +/- 0.05
D); refraction also shifted in a myopic direction by a mean of 0.15 +/
- 0.06 D compared with the high contrast letters. A third experiment w
ith four less-experienced subjects demonstrated the importance of inst
ruction and training in any measurement involving judgment of just-per
ceptible defocus blur. The clinical implications of the results for me
asurements of refraction and amplitude of accommodation are discussed.