Mi. Dobrikov et al., SENSITIZED PHOTOMODIFICATION OF SINGLE-STRANDED-DNA BY A BINARY-SYSTEM OF OLIGONUCLEOTIDE CONJUGATES, ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 7(4), 1997, pp. 309-317
Citations number
32
Categorie Soggetti
Medicine, Research & Experimental","Biothechnology & Applied Migrobiology
A photoactivatable binary system of oligonucleotide conjugates that fo
rm reactive species when assembling on a target nucleotide sequence ha
s been developed, The binary system consists of two oligonucleotides.
One contains a photosensitizing group, and the second contains a photo
reactive group, Binding of the oligonucleotides to adjacent sequences
in the target nucleic acid brings the groups in contact, which allows
transfer of the absorbed energy from the sensitizer to the reagent and
triggers crosslinking of the reagent to the target, One advantage of
the binary system is the improved specificity, which is determined by
independent binding of two oligonucleotides to the target sequence, An
other advantage is the very high efficiency of the reaction achieved b
ecause each molecule of the target-bound sensitizing conjugate can act
ivate many photoreactive oligonucleotide conjugate molecules bound to
the target sequence.