Te. Saisonbehmoaras et al., ANTISENSE PROPERTIES OF END-MODIFIED OLIGONUCLEOTIDES TARGETED TO HA-RAS ONCOGENE, ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 7(4), 1997, pp. 361-368
Citations number
25
Categorie Soggetti
Medicine, Research & Experimental","Biothechnology & Applied Migrobiology
Phosphodiester oligodeoxyribonucleotides linked to an intercalating ag
ent or a dodecanol tail or both complementary to the 12th codon region
of Ha-ras mRNA were compared with the unmodified oligonucleotides of
the same size and sequence with respect to their ability to induce RNa
seH cleavage and antisense activity in cell culture. The hydrophobic t
ail not only protected the oligonucleotide from nucleases but also enh
anced RNase H cleavage of the target. Oligonucleotides carrying both a
n acridine and a dodecanol substituent inhibited the proliferation of
HBL100ras1 cells (human mammary cells stably transformed with the T24
Ha-ras gene carrying a G-->T point mutation in codon 12) at a 20-fold
to 30-fold lower concentration than unmodified ones. Therefore, these
modified oligonucleotides may prove useful for antisense applications.