C. Giovannangeli et C. Helene, PROGRESS IN DEVELOPMENTS OF TRIPLEX-BASED STRATEGIES, ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 7(4), 1997, pp. 413-421
Citations number
128
Categorie Soggetti
Medicine, Research & Experimental","Biothechnology & Applied Migrobiology
Recognition of B-DNA by oligonucleotides that form triple helices is a
unique method to specifically recognize sequences of double-stranded
DNA, Recently, some significant limitations of the triple-based applic
ations have been overcome. Stable intermolecular triplexes can be form
ed under physiologic conditions, Binding affinities of modified oligon
ucleotides to their target sequence due to Hoogsteen or reverse Hoogst
een hydrogen bonding interactions are now in the range of those obtain
ed for duplex formation via Watson-Crick hydrogen bonding interactions
even if the kinetics may be quite different. Progress has been made t
oward developing general procedures to determine the molecular mechani
sms of action of tripler-forming oligonucleotides (TFO) administered t
o cultured cells to provide a rational proof-of-concept for antigene s
trategies, The antigene strategy has reached a point where TFOs can be
used to interfere with several biologic processes (replication, trans
cription, recombination, repair) in relevant systems both in vitro and
ex vivo.