DETERMINATION OF THE INNER SURFACE-POTENTIAL OF THE ERYTHROCYTE-MEMBRANE

Authors
IGLIC A BRUMEN M SVETINA S
Citation
A. Iglic et al., DETERMINATION OF THE INNER SURFACE-POTENTIAL OF THE ERYTHROCYTE-MEMBRANE, Bioelectrochemistry and bioenergetics, 43(1), 1997, pp. 97-103
Citations number
42
Categorie Soggetti
Biology
Journal title
Bioelectrochemistry and bioenergetics → ACNP
ISSN journal
03024598
Volume
43
Issue
1
Year of publication
1997
Pages
97 - 103
Database
ISI
SICI code
0302-4598(1997)43:1<97:DOTISO>2.0.ZU;2-C
Abstract
The time dependent uptake of 1-anilino-8-naphthalene sulphonate (ANS) by bovine erythrocytes was studied. Experimental results were compared with theoretical predictions in order to determine the inner erythroc yte membrane surface potential. The value of the inner membrane surfac e potential under physiological conditions was estimated as -35 mV. In addition, the number of ANS binding sites on the outer surface of the single bovine erythrocyte membrane was determined as 2.2 X 10(6). (C) 1997 Elsevier Science S.A.