Rt. Pon et Sy. Yu, HYDROQUINONE-O,O'-DIACETIC ACID (Q-LINKER) AS A REPLACEMENT FOR SUCCINYL AND OXALYL LINKER ARMS IN SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS, Nucleic acids research, 25(18), 1997, pp. 3629-3635
When hydroquinone-O,O'-diacetic acid is used as a linker arm in solid
phase oligonucleotide synthesis, the time for NH4OH cleavage of oligod
eoxy- or oligoribonucleotides is reduced to only 2 min. This allows in
creased productivity on automated DNA synthesizers without requiring a
ny other modifications to existing reagents or synthesis and deprotect
ion methods, The Q-linker may also be rapidly cleaved by milder reagen
ts such as 5% NH4OH, potassium carbonate, anhydrous ammonia, t-butylam
ine or fluoride ion. However, the Q-linker is sufficiently stable for
longterm storage at room temperature without degradation and no loss o
f material occurs during synthesis, The linker is also reasonably resi
stant to 20% piperidine/DMF, 0.5 M DBU/pyridine and 1:1 triethylamine/
ethanol. The Q-linker can therefore serve as a general replacement for
both succinyl and oxalyl linker arms.