FLP-MEDIATED DNA MOBILIZATION TO SPECIFIC TARGET SITES IN DROSOPHILA CHROMOSOMES

The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination o f gene expression and chromosomal position effects. We report that the FLP-FRT site-specific recombination system of the yeast 2 mu plasmid can be used to integrate DNA at a chromosomal FRT target site in Droso phila. The technique we used was to first integrate an FRT-flanked gen e by standard P element-mediated transformation, FLP was then used to excise the FRT flanked donor DNA and screen for FLP-mediated re-integr ation at an FRT target at a different chromosome location, Such events were recovered from up to 5% of the crosses used to screen for mobili zation and are easily detectable by altered linkage of a white reporte r gene or by the generation of a white(+) gene upon integration.