FLUORESCENCE-LABELING, ISOTOPE-LABELING OR BIOTIN-LABELING OF THE 5'-END OF SINGLE-STRANDED DNA RNA USING T4 RNA LIGASE/

Authors
KINOSHITA Y NISHIGAKI K HUSIMI Y
Citation
Y. Kinoshita et al., FLUORESCENCE-LABELING, ISOTOPE-LABELING OR BIOTIN-LABELING OF THE 5'-END OF SINGLE-STRANDED DNA RNA USING T4 RNA LIGASE/, Nucleic acids research, 25(18), 1997, pp. 3747-3748
Citations number
8
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
25
Issue
18
Year of publication
1997
Pages
3747 - 3748
Database
ISI
SICI code
0305-1048(1997)25:18<3747:FIOBOT>2.0.ZU;2-K
Abstract
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, w hich is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluoresc ence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and p olynucleotides.