INVERSE FILTERING AND LINEAR PREDICTION AS NEW ANALYTICAL APPROACHES TO ANALYZE THE CHANGE OF ABSORBENCY SPECTRA IN VISIBLE SPECTROSCOPY - IN-VIVO STUDY OF PROTOCHLOROPHYLL(IDE) PHOTOTRANSFORMATION

The common approach of analyzing spectra by derivation is known to be inadequate for kinetic studies in visible spectroscopy (R. I. Sharger, Photochem. Photobiol, 38, 615-617, 1983) because the side-lobes heigh t introduces artifacts and reduces readability in three-dimensional gr aphic representation. In this study we present for the first time a pa rametric method that enables the use of inverse filtering and linear p rediction for kinetic investigation in visible spectroscopy in cell bi ology studies. The protochlorophyll(ide) phototransformation at room t emperature (295 K) was used to demonstrate the performance of this ana lytical approach. The change of absorption spectra in etiolated barley leaves was analyzed after being illuminated by a 50 ms flash.