Lb. Poole et al., PEROXIDASE-ACTIVITY OF A TSA-LIKE ANTIOXIDANT PROTEIN FROM A PATHOGENIC AMEBA, Free radical biology & medicine, 23(6), 1997, pp. 955-959
The 29 kDa surface protein of Entamoeba histolytica is an abundant ant
igenic protein expressed by pathogenic strains of this organism. The p
rotein is a member of a widely-dispersed group of homologues which inc
ludes at least two cysteinyl peroxidases, Salmonella typhimurium alkyl
hydroperoxidase C-22 protein (AhpC) and Saccharomyces cerevisiae thio
l-specific antioxidant protein (TSA). Here, for the first time in a pa
thogenic eukaryote, we have demonstrated that the amoebic protein also
possesses peroxidatic and antioxidant activities in the presence of r
eductants such as dithiothreitol or thioredoxin reductase plus thiored
oxin. Although the S. typhimurium AhpF flavoprotein was not an effecti
ve reductant of the amoebic TSA protein, one inhibitory monoclonal ant
ibody directed toward amoebic TSA was also partially inhibitory toward
reduced but not oxidized bacterial AhpC. These antioxidant proteins a
re likely to be important not only in general cell protection, but als
o in the promotion of infection and invasion by these pathogenic organ
isms through protection against oxidative attack by activated host pha
gocytic cells. (C) 1997 Elsevier Science Inc.