Ak. Bhuyan et al., MICROPROPAGATION OF CURRY LEAF TREE [MURRAYA-KOENIGII (L.) SPRENG] BYAXILLARY PROLIFERATION USING INTACT SEEDLINGS, Plant cell reports, 16(11), 1997, pp. 779-782
An efficient and reproducible procedure for the large scale propagatio
n of Murraya koenigii (L.) Spreng. (Curry Leaf Tree) is described. Hig
h-frequency direct shoot proliferation was induced in intact seedlings
of M. koenigii on modified Murashige and Skoog (1962) (MS) medium sup
plemented with 5.0 mg/l benzyladenine. Shoot buds originated from the
region adjacent to the apex of the primary shoot and the epicotyledona
ry node of the intact seedling. Shoots elongated following transfer to
MS medium without plant growth regulators. The shoot-forming capacity
of intact seedlings was influenced by explant orientation. Maximum sh
oot proliferation was obtained when the shoot-forming region was in di
rect contact with the medium surface or slightly embedded into the med
ium. Proliferating shoot cultures were established by repeatedly subcu
lturing mother seedlings on fresh medium of the same composition after
excising all newly formed shoots. Roots were formed on excised shoots
when they were transfered to half-strength MS containing 1.0 mg/l ind
ole-3-butyric acid. Plantlets were acclimatized and established in soi
l where they exhibited normal growth.