In order to study the purity of recycled fibre-based materials, produc
ts containing recycled fibre as well as recycled pulp were examined wi
th regard to their chemical impurities, toxicity and microbiological q
uality. The study was carried out to clarify both qualitatively and qu
antitatively the variations in microbiological quality. The levels of
several classes of chemical substances were analysed and semi-volatile
and volatile substances identified. The toxicity and mutagenicity of
virgin fibre and recycled fibre materials were screened using the Phot
obacter toxicity test and the Ames Salmonella mutagenicity test. Preli
minary chemical characterization of the mutagens was carried out. Iden
tification of the compounds found in the mutagenic fractions was perfo
rmed by gas chromatography/mass spectrometry (GC/MS). The concentratio
n of various substances analysed was found to be low, although the var
iety of substances present appeared to be very broad. Preliminary chem
ical characterization revealed that some samples contained compounds k
nown to have mutagenic or other toxic activity. Also, the recycled fib
re pulps contained large amounts of various microbes, the microbial lo
ad consisting mainly of aerobic spore-forming bacteria. The paper-maki
ng process was found to clearly have reduced the total microbial count
s.