RESOLUTION OF DL-AMINO ACIDS ON A NATIVE CELLULOSE COLUMN AND A PLAUSIBLE MECHANISM FOR THEIR RESOLUTION

We have studied the resolution of DL-amino acids on a native cellulose column, All the DL-amino acids related to protein and their 16 DNP-DL -amino acids were separated. The resolution capability depends mainly upon the bulkiness of the side group attached to the alpha-carbon, but also on structural and functional interaction of amino acid with cell ulose, We propose a plausible resolution mechanism that is thought to be governed by a so-called key and lock relation between an amino acid and cellulose. Then, the affinity of each enantiomer for cellulose wa s calculated based on the resolution factor, which was known to be som e 10(-2)-10(-3) eV. (C) 1997 by John Wiley & Sons, Ltd.