ELECTROKINETIC SEPARATION OF ENANTIOMERS USING A CAPILLARY COATED WITH POLY-TERGURIDE

Enantioseparation of 2-arylpropionic acids (Flobufen) and dansyl-deriv atives of amino acids can be achieved in 10-15 min by electrochromatog raphy using a capillary coated with poly-terguride, It was found that the analytes, in the range of the buffer pH between 2.5-4.0, were driv en by anodic electroosmotic flow originated by the positively charged moieties of the ergolinic skeleton, and, only partially, by their anod ic electrophoretic mobility, The enantiomers eluted with the same sequ ence, e.g. L-or (S)-isomer more retained than D-or (R)-, which was obs erved in HPLC separations on terguride-silica based CSPs, thus indicat ing that racemates were resolved by a similar chiral recognition mecha nism, The retention of the solutes was affected by the concentration a nd composition of the effluent, An other mobile phase variable, strong ly affecting the selectivity, was the pH of the buffer. (C) 1997 by Jo hn Wiley & Sons, Ltd.