SEPARATION OF BENZODIAZEPINES ON A NEW CARBOHYDRATE-BASED CHIRAL STATIONARY-PHASE FOR HPLC

Several benzodiazepines were enantioresolved on a new carbohydrate chi ral stationary phase based on maltooligosaccharides. The role of organ ic modifier, ionic strength, pH and temperature are examined and the r esults are discussed, In general, selectivity and retention were found to decrease with increasing organic modifier concentration. The appea rance of two peaks for diazepam suggests that the reported separations are based on separation of conformers and was supported using circula r dichroism detection. Counterintuitively, resolution and enantioselec tivity were found to improve with increasing temperatures. (C) 1997 by John Whey & Sons, Ltd.