FLOW-INJECTION CHEMILUMINESCENT DETERMINATION OF GLYCEROL-3-PHOSPHATEAND GLYCEROPHOSPHORYLCHOLINE USING IMMOBILIZED ENZYMES

Authors
YAQOOB M NABI A MASOOMYASINZAI M
Citation
M. Yaqoob et al., FLOW-INJECTION CHEMILUMINESCENT DETERMINATION OF GLYCEROL-3-PHOSPHATEAND GLYCEROPHOSPHORYLCHOLINE USING IMMOBILIZED ENZYMES, Journal of bioluminescence and chemiluminescence, 12(1), 1997, pp. 1-5
Citations number
19
Categorie Soggetti
Biology
Journal title
Journal of bioluminescence and chemiluminescence → ACNP
ISSN journal
08843996
Volume
12
Issue
1
Year of publication
1997
Pages
1 - 5
Database
ISI
SICI code
0884-3996(1997)12:1<1:FCDOG>2.0.ZU;2-0
Abstract
Flow injection procedures with immobilized enzyme mini-columns are des cribed for the determination of glycerol-3-phosphate, and glycerophosp horylcholine with chemiluminescent detection. The hydrogen peroxide pr oduced on-line is coupled with a luminol (5-amino-2,3,-dihydro-1,4-pht halazinedione) peroxidation chemiluminescent system. The detection lim its for glycerol-3-phosphate and glycerophosphorylcholine are 5x10(-7) M and 1x10(-6)M respectively with r.s.d. <2%. The sample throughput i s 40/h. The immobilized enzyme columns did not show any deterioration in activity after usage for 3 months. (C) 1997 John Wiley & Sons, Ltd.