CHARACTERIZATION OF MICROSOMAL GST-II BY WESTERN-BLOT AND IDENTIFICATION OF A NOVEL LTC4 ISOMER

Authors
JAKOBSSON PJ SCOGGAN KA YERGEY J MANCINI JA FORDHUTCHINSON AW
Citation
Pj. Jakobsson et al., CHARACTERIZATION OF MICROSOMAL GST-II BY WESTERN-BLOT AND IDENTIFICATION OF A NOVEL LTC4 ISOMER, Journal of lipid mediators and cell signalling, 17(1), 1997, pp. 15-19
Citations number
7
Categorie Soggetti
Biology,"Cell Biology
Journal title
Journal of lipid mediators and cell signalling → ACNP
ISSN journal
09297855
Volume
17
Issue
1
Year of publication
1997
Pages
15 - 19
Database
ISI
SICI code
0929-7855(1997)17:1<15:COMGBW>2.0.ZU;2-I
Abstract
Protein expression of microsomal GST-II and LTC4 synthase was analyzed by Western blot. Correlation between a 17 kDa band and LTC, formation was observed for both enzymes. The expression of microsomal GST-II wa s several fold more efficient than the expression of LTC4 synthase. In addition to catalyzing the biosynthesis of LTC4, microsomal GST-II al so produces another product, which has been subjected to mass spectrom etric analysis. This analysis demonstrates that the novel product is a n isomer of LTC4. (C) 1997 Elsevier Science B.V.