CHARACTERIZATION OF THE MENINGOCOCCAL TRANSFERRIN-BINDING PROTEIN COMPLEX BY PHOTON-CORRELATION SPECTROSCOPY

Authors
BOULTON IC GORRINGE AR CARR RJG GORINSKY B JOANNOU CL EVANS RW
Citation
Ic. Boulton et al., CHARACTERIZATION OF THE MENINGOCOCCAL TRANSFERRIN-BINDING PROTEIN COMPLEX BY PHOTON-CORRELATION SPECTROSCOPY, FEBS letters, 414(2), 1997, pp. 409-413
Citations number
22
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
414
Issue
2
Year of publication
1997
Pages
409 - 413
Database
ISI
SICI code
0014-5793(1997)414:2<409:COTMTP>2.0.ZU;2-V
Abstract
Photon correlation spectroscopy demonstrated for the first time that c o-purified meningococcal TbpA+B form a complex in solution. This struc ture bound hTf and the resultant species under,vent partial dissociati on after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar apparent sizes but showed distinct ive size profiles suggesting that TbpA forms a largely homogeneous pop ulation while TbpB may produce more variable particle sizes under thes e conditions. (C) 1997 Federation of European Biochemical Societies.